Methionine-Choline Deficient Diet Relieves Dextran Sodium Sulfate-Induced Colitis via Maintenance of Non Type Ⅰ NKT Cells

Sagami, Shintaro

Thursday, July 16, 2015

Grand Hall and Gallery, Ground Floor & 1st Floor (Maritim Hotel)

Shintaro Sagami, MD , Departments of Gastroenterology, Hiroshima University, Hiroshima, Japan

Yoshitaka Ueno, PhD , Endoscopy, Hiroshima University, Hiroshima, Japan

Shinji Tanaka, PhD , Endoscopy, Hiroshima University, Hiroshima, Japan

Soki Nishiyama, MD , Departments of Gastroenterology and Metabolism, Hiroshima University, Hiroshima, Japan

Ryohei Hayashi, MD , Departments of Gastroenterology and Metabolism, Hiroshima University, Hiroshima, Japan

Kenta Nagai, MD , Departments of Gastroenterology and Metabolism, Hiroshima University, Hiroshima, Japan

Shiro Oka, PhD , Endoscopy, Hiroshima University, Hiroshima, Japan

Toru Hiyama, PhD , Health Service Center, Hiroshima University, Higashihiroshima, Japan

Masanori Ito, PhD , Departments of Gastroenterology and Metabolism, Hiroshima University, Hiroshima, Japan

Yasuhiko Kitadai, PhD , Departments of Gastroenterology and Metabolism, Hiroshima University, Hiroshima, Japan

Masaharu Yoshihara, PhD , Health Service Center, Hiroshima University, Higashihiroshima, Japan

Kazuaki Chayama, PhD , Departments of Gastroenterology and Metabolism, Hiroshima University, Hiroshima, Japan

Dietary choline is important for the prevention of tissue oxidative damage and ER stress. However, little is known about the role of choline in intestinal homeostasis. In the present study, we investigated the effect of choline deficiency in intestinal immunity in a mouse model of dextran sodium sulfate (DSS)-induced colitis. C57BL/6(B6) mice and Jα18 deficient (KO) mice were fed methionine-choline deficiency diet (MCD) or control food (CF) for 4 weeks before the induction of DSS colitis. Body weight change, colon length, and histological damage in the colon were examined. Characterization of hepatic NK1.1⁺ T cells was assessed by fluorescence-activated cell sorting analysis (FACS) analysis. Oral administration of MCD facilitates the body weight loss and shortening of colon length associated with DSS-induced colitis on B6 mice. Histological analysis also revealed that the colonic inflammation was exacerbated by MCD. However, the colitis on KO mice was milder with MCD administration. FACS analysis revealed that NK 1.1⁺ T cells in the liver were significantly decreased in MCD-fed B6 mice. On the other hand, there are no significant differences in the number of NK1.1⁺ T cells between CF and MCD-fed KO mice. Even 1-week feeding with MCD exaggerated colitis in B6, but not KO mice. These results suggest that choline deficiency may maintain non type Ⅰ NKT cells which improve colitis.

ICMI 2015

T.39 Methionine-Choline Deficient Diet Relieves Dextran Sodium Sulfate-Induced Colitis via Maintenance of Non Type Ⅰ NKT Cells