Thursday, July 16, 2015
Grand Hall and Gallery, Ground Floor & 1st Floor (Maritim Hotel)
Guillermo Docena
,
Department of Biological Sciences, LA PLATA, Buenos Aires, Argentina
Maria Lucia Orsini Delgado
,
University of La Plata, La Plata, Argentina
Alicia Sambuelli
,
Gastroenterology Hospital Dr Udaondo, Buenos Aires, Argentina
Aníbal Gil
,
Gastroenterology Hospital Dr Udaondo, Buenos Aires, Argentina
Silvia Negreira
,
Gastroenterology Hospital Dr Udaondo, Buenos Aires, Argentina
Sergio Huernos
,
Gastroenterology Hospital Dr Udaondo, Buenos Aires, Argentina
Silvina Goncalves
,
Gastroenterology Hospital Dr Udaondo, Buenos Aires, Argentina
Maricel Bellicoso
,
Gastroenterology Hospital Dr Udaondo, Buenos Aires, Argentina
Pablo Tirado
,
Gastroenterology Hospital Dr Udaondo, Buenos Aires, Argentina
Nicolas Enrique
,
University of La Plata, La Plata, Argentina
Veronica Milesi
,
University of La Plata, La Plata, Argentina
Air pollution containing particulate matter and industrial pollutants has been related with Inflammatory Bowel Disease (IBD) early debut and hospitalization. Volcanic pollution has not been studied in IBD, even in highly exposed regions with high prevalence of IBD. We hypothesised that during the prolonged Puyehue eruptions (2011), volcanic ash (VA) exerted a pro-inflammatory effect promoting IBD refractoriness. We here studied the inflammatory effect of VA added to the drinking water in a colitis model.
BALB/c mice receiving drinking water with or without VA, (grouped w/ or w/o VA) were intrarectally administered with inflammatory stimuli (TNBS or flagellin) or ethanol (as control) at day 7. Weight was daily monitored, and at day 14 animals were sacrificed. Colon was removed and studied: weight, length, histology, ZO-1 distribution (confocal microscopy), cytokine and chemokine expression (qPCR) and, acetylcholine-induced colon contractility.
Animals w/VA plus stimuli showed a greater weight loss, colonic weight/length ratio, DAI, HAI (with cellular infiltration, haemorrhage/vascularisation), TNF-α, Ccl20, T-bet, IFN-γ mRNA levels significantly increased (p<0,05), diminished ZO-1 (reflecting barrier impairment), and significantly-increased colon contractile activity.
In conclusion, oral VA administration exerted a pro-inflammatory effect, exacerbating mice colitis. These findings could be related with the observed IBD worsening during volcanic particulate matter exposure.
