Friday, July 17, 2015
Grand Hall and Gallery, Ground Floor & 1st Floor (Maritim Hotel)
Attaching and effacing pathogens, including enterohemorrhagic Escherichia coli in humans and Citrobacter rodentium in mice, raise serious public health concerns. Here we demonstrate that interleukin-1 receptor (IL-1R) signaling is indispensable for protection against C. rodentium infection in mice. Oral challenge with C. rodentium led to severe loss of body weight and high mortality in IL-1R-/- mice at 7–14 days after infection whereas wild-type mice recovered from mild symptoms within 3 weeks. At day 10 after infection, mRNA and protein levels of KC/CXCL1 were significantly reduced in colon homogenates of infected IL-1R-/- mice as compared with wild-type one. Of note, infiltration of IL-22-secreting CD11b+Ly6C+Ly6G+ cells was significantly defective in the colons of IL-1R-/- mice at day 4 after infection. Of most interest, colonic stromal cells isolated from IL-1R-/- mice secreted lower levels of KC/CXCL1 than stromal cells from wild-type mice during C. rodentium infection. Similar effects were found when mouse colonic stromal cells and human nasal polyp stromal cells were treated with IL-1R antagonists (i.e., anakinra) in vitro. These results suggest that IL-1 signaling plays a pivotal role in activating mucosal stromal cells to secrete chemokines, which are essential for infiltration of innate immune cells upon bacterial infection.