Here we have shown that the TGFβ-activating integrin αvβ8, which we have previously found to play an important role in murine intestinal immune homeostasis, is preferentially expressed on human CD1c+ intestinal dendritic cells (DC). Interestingly, expression on these cells is increased during pathological inflammation. We find that expression of integrin αvβ8 is upregulated on human DC after LPS stimulation, suggesting the enhanced expression of αvβ8 during inflammation could be driven by an infiltrating microbiota. Importantly, we have also shown that TGFβ activation by αvβ8 on human DC promotes CD25+CD127- Foxp3+ Treg induction ex vivo. Together, our data suggest that integrin αvβ8-mediated TGFβ activation by DC may play a role in the regulation of T cell responses in the human intestine, and that this pathway may be perturbed during intestinal inflammation.