Friday, July 17, 2015
Grand Hall and Gallery, Ground Floor & 1st Floor (Maritim Hotel)
Marijana Basic
,
Hannover Medical School, Institute for Laboratory Animal Science, Hannover, Lower Saxony, Germany
Manuela Buettner
,
Hannover Medical School, Institute for Laboratory Animal Science, Hannover, Germany
Lydia Keubler, PhD
,
Institute of Laboratory Animal Science, Hannover, Germany
Anna Smoczek
,
Hannover medical school, Institute of Laboratory Animal Science, Hannover, Germany
André Bleich
,
Hannover Medical School, Institute for Laboratory Animal Science, Hannover, Germany
Noroviruses are highly prevalent RNA viruses which infect gastrointestinal tract of different mammalian species. Murine noroviruses (MNV) are highly prevalent in mouse colonies worldwide. Recently was shown that MNV is capable of inducing histopathological changes in conventionaly housed mice. However, the underlying mechanism of the inflammation development is not well understood yet. Altered Schaedler Flora (ASF) is a defined microbiota community composed from eight bacterial species. In this study we analyzed the influence of MNV on immunological and histological characteristic of intestinal inflammation in germ-free (GF) and ASF colonized IL10-deficient (Il10
-/-) mice.
In the present study GF and ASF colonized B6-Il10-/- mice were monitored for structural and functional intestinal barrier alterations by histology, TUNEL staining, qRT-PCR and ELISA.
Intestinal inflammation was not observed in GF and MNV infected Il10-/- mice, but the histological score increased after ASF colonization and was even higher after MNV infection. Gene expression of tight junction proteins was not changed after MNV infection, but it was changed within 4 weeks in MNV infected mice colonized with ASF. Mice colonized with ASF and infected with MNV showed 4 weeks post infection increased production of IFNg and IgA.
MNV can induce mucosal inflammation and changes in intestinal epithelial barrier in the susceptible host in the presence of defined intestinal flora.
