ICMI 2015

F.64 Identification and characterization of the “GUT VASCULAR BARRIER” that is exploited by Salmonella typhimurium for its systemic dissemination

Friday, July 17, 2015
Grand Hall and Gallery, Ground Floor & 1st Floor (Maritim Hotel)
Ilaria Spadoni, PhD , European Institute of Oncology, Milan, MI, Italy
Elena Zagato, PhD , European Institute of Oncology, Milan, Italy
Alice Bertocchi , European Institute of Oncology, Milan, Italy
Roberta Paolinelli, PhD , 2IFOM, FIRC Institute of Molecular Oncology, Milan, Italy
Antonio Di Sabatino, MD , 3First Department of Medicine, St. Matteo Hospital, University of Pavia, Pavia, Italy
Flavio Caprioli, MD PhD , Ospedale Maggiore Policlinico di Milano, Milan, Italy
Luca Bottiglieri, MD , European Institute of Oncology, Milan, Italy
Amanda Oldani, PhD , IFOM, FIRC Institute of Molecular Oncology, Milan, Italy
Giuseppe Viale, MD , European Institute of Oncology, Milan, Italy
Giuseppe Penna , European Institute of Oncology, Milan, Italy
Elisabetta Dejana, PhD , IFOM, FIRC Institute of Molecular Oncology, Milan, Italy
Maria Rescigno, Prof , European Institute of Oncology, Milano, Italy
Orally administered antigens can reach systemic sites inducing a systemic state of tolerance, while the microbiota is contained in the mucosal compartment and is systemically ignored. How this discrimination is achieved is unknown.We describe the existence of a gut vascular barrier (GVB) both in human and mouse that plays an important role in controlling the translocation of bacteria to systemic sites. We characterized the intestinal endothelial cells (ECs) in terms of expression of tight and adherent junction proteins. In addition, we observed that the ECs were associated with enteric glial cells and pericytes forming together the “gut vascular unit”.GVB integrity could be modified by Salmonella typhimurium. Indeed, upon infection the vascular ECs up-regulated the expression of PV1, marker of damaged vascular barriers, which correlated with higher endothelial permeability.S. typhimurium could modify barrier properties of the ECs through the negative regulation of the Wnt/β-catenin signaling pathway. Indeed, we found that β-catenin activation was reduced upon infection in vitro. Consistently, Salmonella was incapable to modify ECs permeability and to spread systemically in mice where β-catenin was constitutively activated by genetic means in vascular ECs. Furthermore, we demonstrated that Salmonella pathogenicity island (spi)-2 was involved in the regulation of Wnt/β-catenin signaling pathway.