Specific Serum IgG Subclass Antibodies Against Food and Microbial Antigens in Inflammatory Bowel Diseases Patients

Jansen, Anke

Thursday, July 16, 2015

Grand Hall and Gallery, Ground Floor & 1st Floor (Maritim Hotel)

Anke Jansen , Department of Internal Medicine III, University Hospital RWTH Aachen, Aachen, Germany

Ana Mandic , Uniklinik Aachen, Aachen, NW, Germany

Eveline Bennek , Department of Internal Medicine III, University Hospital RWTH Aachen, Aachen, Germany

Lisa Frehn, MD , Department of Internal Medicine III, University Hospital RWTH Aachen, Aachen, Germany

Julien Verdier, PhD , Department of Internal Medicine III, University Hospital RWTH Aachen, Aachen, Germany

Irene Tebrügge, MD , Department of Internal Medicine III, University Hospital RWTH Aachen, Aachen, Germany

Holger Lutz, MD , Department of Internal Medicine III, University Hospital RWTH Aachen, Aachen, Germany

Konrad Streetz, MD , Department of Internal Medicine III, University Hospital RWTH Aachen, Aachen, Germany

Christian Trautwein, MD , Department of Internal Medicine III, University Hospital RWTH Aachen, Aachen, Germany

Gernot Sellge, MD, PhD , University Hospital RWTH Aachen, Aachen, Germany

Background:Inflammatory bowel disease (IBD), particularly Crohn's disease (CD), is associated with increased microbial-specific IgG and IgA antibodies, whereas alterations of anti-food antibodies are still disputed. The knowledge about IgG subclass antibodies in IBD is limited.

Methods: Serum IgG1, IgG2, IgG3 and IgG4 specific for nutritional and microfloral antigens (wheat and milk extracts; purified ovalbumin; Escherichia coli and Bacteroides fragilis lysates; mannan from Saccharomyces cerevisiae) were analysed by ELISA in patients with CD (n = 56), ulcerative colitis (UC; n = 29), acute gastroenteritis/colitis (n = 12) as well as non-inflammatory controls (n = 62).

Results: Anti-Saccharomyces cerevisiae antibodies (ASCA) of all IgG subclasses and anti-B. fragilis IgG1 levels were increased in CD patients compared to UC patients and controls. The discriminant validity of ASCA IgG2 and IgG4 was comparable to that of ASCA pan-IgG and IgA, whereas it was inferior for ASCA IgG1/IgG3 and anti-B. fragilis IgG1. Stricturing/penetrating disease behaviour in CD patients was significantly associated with increased ASCA IgG1/IgG3/IgG4, anti-B. fragilis IgG1 and anti-E. coli IgG1 levels. Conversely, anti-TNF-α treatment in CD patients was associated with reduced ASCA IgG1/IgG2/IgG4, anti-B. fragilis IgG1 and anti-E. coliIgG1. Several anti-food IgG subclass concentrations were decreased in IBD patients with arthropathy compared to those without joint involvement. Anti-food IgG subclass levels did not correlate with food intolerance.

Conclusion:Several microbial-specific but not food-specific IgG subclasses are increased in CD patients. The relevance of decreased anti-food IgG subclass levels in IBD patients with arthropathy remains unclear and requires further investigation

ICMI 2015

T.116 Specific Serum IgG Subclass Antibodies Against Food and Microbial Antigens in Inflammatory Bowel Diseases Patients