ICMI 2015

OR.55 Microbiota Modulates Host Response to Dietary Gluten in NOD/DQ8 Mice.

Thursday, July 16, 2015: 4:00 PM
Salon Dublin, Second Floor (Maritim Hotel)
Heather Galipeau , Farncombe Family Digestive Health Research Institute, McMaster University, Hamilton, ON, Canada
Justin McCarville , Farncombe Family Digestive Health Research Institute, McMaster University, Hamilton, ON, Canada
Alberto Caminero Fernandez , Farncombe Family Digestive Health Research Institute, McMaster University, Hamilton, ON, Canada
Sina Huebener , Department of Medicine, Columbia University Medical Center, New York, NY
Owen Litwin , Farncombe Family Digestive Health Research Institute, McMaster University, Hamilton, ON, Canada
Marlies Meisel , Department of Medicine, University of Chicago, Chicago, IL
Bana Jabri , Department of Medicine, University of Chicago, Chicago, IL
Joseph Murray , Division of Gastroenterology and Hepatology, and Department of Immunology, Mayo Clinic College of Medicine, Rochester, MN
Manel Jordana , Departments of Pathology and Molecular Medicine, McMaster Immunology Research Centre, McMaster University, Hamilton, ON, Canada
Armin Alaedini , Department of Medicine, Columbia University Medical Center, New York, NY
Fernando Chirdo , Instituto de Estudios Inmunologicos y Fisiopatologicos- IIFP, La Plata, Argentina
Elena Verdu , Farncombe Family Digestive Health Research Institute, McMaster University, Hamilton, ON, Canada
Celiac disease (CD) is an immune-mediated enteropathy triggered by the ingestion of gluten in genetically susceptible individuals expressing the HLA-DQ2 or DQ8 genes. Dysbiosis has been described in patients with CD, but it is unknown whether these microbial changes are a CD-promoting factor. We therefore took a gnotobiotic approach to investigate the influence of the microbiota on host responses to gluten in adult NOD/DQ8 mice, a model of gluten-sensitivity. Both conventional specific pathogen free mice, which harbour opportunistic bacteria including Proteobacteria, and germ-free mice developed gluten-induced small intestinal inflammation, characterized by increased intraepithelial lymphocytes, decreased villous-to-crypt ratios, and gluten specific immune responses. However, mice colonized with a limited, defined microbiota devoid of opportunistic pathogens and Proteobacteria (altered Schaedler flora; ASF) were protected from gluten-induced small intestinal inflammation and gluten-specific immune responses. Protection in ASF-colonized mice was reversed by colonization with Pseudomonas aeruginosa, a member of the Proteobacteria phylum, isolated from the small intestine of an active CD patient. These results provide evidence of modulation of host responses to gluten by intestinal microbiota. The presence or absence of specific opportunistic pathogens may promote or prevent gluten-induced inflammation in a genetically susceptible host.