We hypothesized that a diet rich in the two dietary fibers, arabinoxylan and resistant starch, would strengthen the colonic defense barrier evaluated by increased occludin and MUC2 transcription and decreased fecal calprotectin, a marker of gut neutrophil infiltration.
Methods:Nineteen subjects with MetS completed a randomized crossover study with two 4-week diet interventions encompassing a healthy-carbohydrate diet (HCD) rich in arabinoxylan and resistant starch and a low-fiber western style diet. Before and after each intervention we performed endoscopy with biopsies and collected stool samples.
Results: The healthy-carbohydrate diet changed the gut microbiome and most distinctly enhanced Bifidobacterium (p<0.01). Colonic transcription of occludin increased by 17% (p=0.03) and MUC2 by 21% (p=0.02) after HCD, whereas fecal calprotectin decreased by 30% (p<0.05).
Conclusion: Consumption of a diet rich in arabinoxylan and resistant starch for four weeks modified the gut microbiome, increased colonic occludin and MUC2 transcription and decreased fecal calprotectin suggesting a reinforced colonic defense barrier in subjects with MetS.