ICMI 2015

OR.90 In Utero Lymphotoxin Signalling Is Required for Mucosal B Cell Function in Adults

Friday, July 17, 2015: 3:45 PM
Salon 7, Ground Floor (Maritim Hotel)
Conglei Li, MD, PhD , University of Toronto, Toronto, ON, Canada
Lesley Ward, MSc. , Department of Immunology, University of Toronto, Toronto, ON, Canada
Tian Sun, BSc. , Department of Immunology, University of Toronto, Toronto, ON, Canada
Alberto Martin, PhD , Department of Immunology, University of Toronto, Toronto, ON, Canada
Jennifer Gommerman, PhD , Department of Immunology, University of Toronto, Toronto, ON, Canada
Lymphotoxin-beta Receptor (LTβR) signalling is required for development of secondary lymphoid organs, including Peyer’s patches. Serum IgA levels are also severely reduced in LT-deficient mice. While it has been shown that B cell migration to the lamina propria is impaired in LT-deficient mice, it is unknown whether LTβR signalling affects mucosal B cell function. Here we show that expression of LTαβ ligand on hemapoietic cells was dispensable for induction and maintanence of gut B cell response against a mucosal rotavirus infection in mice where LTβR signaling was intact in utero. However, expression of LTαβ ligand on hemapoietic cells was required for optimal mucosal B cell responses in mice where LTβR signaling was absent in utero. Specifically, compared to WT→WT chimeras, AID induction in mucosal B cells was impaired in WT→LTβ-/- chimeric mice concomitant with reduced global fecal IgA levels as well as weaker, slower and non-persistent fecal anti-rotavirus IgA responses. Furthermore, mice treated in utero with LTβR inhibitors also exhibited attenuated mucosal anti-rotavirus responses and alterations in gut resident stromal cells in adulthood. Thus, in utero LTβR signaling shapes gut stromal cell populations and has an impact on mucosal B cell function in adult mice.