ICMI 2015

W.69 Preventive and therapeutic effects of dietary omega3 fatty acid-originated 17,18-epoxyeicostetraenoic acid on the control of intestinal allergy

Wednesday, July 15, 2015
Grand Hall and Gallery, Ground Floor & 1st Floor (Maritim Hotel)
Jun Kunisawa, PhD , The University of Tokyo, Tokyo, Japan
Makoto Arita, PhD , PREST, JST, Tokyo, Japan
Takahiro Hayasaka, PhD , Hamamatsu University School of Medicine, Shizuoka, Japan
Takashi Harada, MD, PhD , Hamamatsu University School of Medicine, Shizuoka, Japan
Ryo Iwamoto, PhD , The University of Tokyo, Tokyo, Japan
Takahiro Nagatake, PhD , National Institute of Biomedical Innovation, Osaka, Japan
Hidehiko Suzuki , National Institute of Biomedical Innovation, Osaka, Japan
Yosuke Kurashima , CREST, JST, Tokyo, Japan
Hiroyuki Arai, PhD , CREST, JST, Tokyo, Japan
Mitsutoshi Setou, MD, PhD , Hamamatsu University School of Medicine, Shizuoka, Japan
Hiroshi Kiyono , CREST, JST, Tokyo, Japan
Omega-3 polyunsaturated fatty acids (PUFAs) have anti-allergic properties, but the metabolic progression from dietary oil to host immune system remains to be elucidated. Here we show that 17, 18-epoxyeicosatetraenoic acid (17,18-EpETE) as an anti-allergy metabolite generated in the colon preferentially from dietary omega-3 alpha-linolenic acid (ALA). Incidence of allergic diarrhea was decreased in mice fed with ALA-rich linseed oil (Lin-mice) when compared with mice fed with conventional soybean oil. MALDI-IMS-based imaging analyses revealed increase of ALA and its metabolites, especially eicosapentaenoic acid (EPA), in the colon of Lin-mice. Further, LC-MS/MS-based mediator lipidomics identified the increase of EPA-derived metabolites including 17,18-EpETE in the colon of Lin-mice. Administration of synthetic 17,18-EpETE showed preventive and therapeutic effects on the development of allergic diarrhea. These data suggest that metabolizing dietary w3 PUFAs generates 17, 18-EpETE, which is an endogenous anti-allergic metabolite and potentially is a therapeutic agent to control intestinal allergic diseases.