Interference of the continuous administration of active VITAMIN D in model of antigen-especific intestinal inflamation.

Although the immune response to food proteins, usually results in tolerance, which is characterized by local hyperactivity with the production of IgA and non-inflammatory cytokines, concomitant with a low systemic production of specific IgG and IgE, proteins routinely tolerated can also induce local inflammatory responses. Aim: evaluate the immunomodulatory role of vitamin D in an antigen specific inflammatory gut model. Methods: Male C57BL/6 mice were subcutaneously administered with vitamin D while being submitted to allergy induction protocol that consisted in the immunized with 100μg of peanut protein. The sensitized groups received a 30-day raw-peanut-challenge-diet (inflamed - I) while the negative control group received mouse chow (controls - C). During the experimental protocol both groups received continuous administration of 75ng, 150ng or 300ng of vitamin D. Results: vitamin D decreased the expected body weight loss in sensitized/inflamed groups, which remained similar to the negative control group C(-). During the same period, there was no significant differences between control and experimental groups in food consumption. With regard to serology, the sensitized groups presented higher IgG titers when compared to negative control group (p<0,01) Furthermore, the solution with vitamin D did not affect the TGO/AST and TGP/ALT values. The results for flow cytometry showed that Vitamin D apparently has a modulatory effect on subpopulation of regulatory T cells when the treated groups were compared to the positive control group. Conclusions: the continuous administration of vitamin D during the experimental period can modify the pattern of total IgG titers.