Wednesday, July 15, 2015
Grand Hall and Gallery, Ground Floor & 1st Floor (Maritim Hotel)
José María Lemme Dumit, Biochemistry
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Cátedra de Inmunología, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, San Miguel de Tucumán, Argentina
Gabriela Perdigon, PhD
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Centro de Referencia para Lactobacilos (CERELA)-CONICET, San Miguel de Tucumán, T, Argentina
Carolina Maldonado Galdeano, PhD
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Cátedra de Inmunología, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, San Miguel de Tucumán, Argentina
Cell wall (CW) molecules from probiotic are crucial to interact through host-receptors and induce signaling that result in the immune system modulation. The knowledge of bacterial components as immunomodulators offers interesting opportunities to elucidate a novel structure as oral adjuvant. In the current study we aimed to study the impact of CW from
Lactobacillus casei CRL431 (Lc431) in the activation of intestinal epithelial cell (IEC) and peritoneal macrophages (MQP) to be the sentinels of innate immune cells.
CW fraction was obtained from Lc431 (CW431). Ex vivo, phagocytic and antimicrobial activities of MQP were determined. In vitro, IL-6 levels of supernatant of MQP were determined. IEC activation was observed trough transmission-electron-microscopy (TEM).
Lc431 and CW431 groups showed phagocytic and microbicidal activity significantly higher in those groups when compared with the control mice, while values for IL-6 do not improved respect to control group. However, Lc431 and CW431 showed for TEM a higher activation of IEC after 30 min of stimulation when compared with IEC control.
Probiotic are able to activate and improve the activity of IEC that is a key cell in mucosal immune system activation. This work demonstrated that cell wall fraction, could be interesting alternative as oral mucosal immunomodulators.
